LOCUS       40924_48328        3666 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pZA3PLtetO-1 luc, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Lutz R, Bujard H.
  TITLE     Independent and tight regulation of transcriptional units in 
            Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 
            regulatory elements
  JOURNAL   Nucleic Acids Res. 25 (6), 1203-1210 (1997)
  PUBMED    9092630
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   Lutz R, Bujard H.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-AUG-1996) Zentrum fuer Molekulare Biologie, University
            of Heidelberg, Im Neuenheimer Feld 282, Heidelberg 69120, Germany
REFERENCE   3  (bases 1 to 3666)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3666)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "1997"; volume: "25"; issue: "6"; pages: 
            "1203-1210"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (07-AUG-1996) Zentrum fuer Molekulare Biologie, University of 
            Heidelberg, Im Neuenheimer Feld 282, Heidelberg 69120, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3666
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        7..80
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator
                     sites (Lutz and Bujard, 1997)"
     RBS             90..101
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             108..1757
                     /label=luciferase
                     /note="firefly luciferase"
     terminator      1776..1862
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(2032..2577)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     terminator      complement(2691..2785)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2809..3465)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(3466..3568)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
ORIGIN
        1 ctcgagtccc tatcagtgat agagattgac atccctatca gtgatagaga tactgagcac
       61 atcagcagga cgcactgacc gaattcatta aagaggagaa aggtaccatg gaagacgcca
      121 aaaacataaa gaaaggcccg gcgccattct atccgctaga ggatggaacc gctggagagc
      181 aactgcataa ggctatgaag agatacgccc tggttcctgg aacaattgct tttacagatg
      241 cacatatcga ggtgaacatc acgtacgcgg aatacttcga aatgtccgtt cggttggcag
      301 aagctatgaa acgatatggg ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact
      361 ctcttcaatt ctttatgccg gtgttgggcg cgttatttat cggagttgca gttgcgcccg
      421 cgaacgacat ttataatgaa cgtgaattgc tcaacagtat gaacatttcg cagcctaccg
      481 tagtgtttgt ttccaaaaag gggttgcaaa aaattttgaa cgtgcaaaaa aaattaccaa
      541 taatccagaa aattattatc atggattcta aaacggatta ccagggattt cagtcgatgt
      601 acacgttcgt cacatctcat ctacctcccg gttttaatga atacgatttt gtaccagagt
      661 cctttgatcg tgacaaaaca attgcactga taatgaactc ctctggatct actgggttac
      721 ctaagggtgt ggcccttccg catagaactg cctgcgtcag attctcgcat gccagagatc
      781 ctatttttgg caatcaaatc attccggata ctgcgatttt aagtgttgtt ccattccatc
      841 acggttttgg aatgtttact acactcggat atttgatatg tggatttcga gtcgtcttaa
      901 tgtatagatt tgaagaagag ctgtttttac gatcccttca ggattacaaa attcaaagtg
      961 cgttgctagt accaacccta ttttcattct tcgccaaaag cactctgatt gacaaatacg
     1021 atttatctaa tttacacgaa attgcttctg ggggcgcacc tctttcgaaa gaagtcgggg
     1081 aagcggttgc aaaacgcttc catcttccag ggatacgaca aggatatggg ctcactgaga
     1141 ctacatcagc tattctgatt acacccgagg gggatgataa accgggcgcg gtcggtaaag
     1201 ttgttccatt ttttgaagcg aaggttgtgg atctggatac cgggaaaacg ctgggcgtta
     1261 atcagagagg cgaattatgt gtcagaggac ctatgattat gtccggttat gtaaacaatc
     1321 cggaagcgac caacgccttg attgacaagg atggatggct acattctgga gacatagctt
     1381 actgggacga agacgaacac ttcttcatag ttgaccgctt gaagtcttta attaaataca
     1441 aaggatacca ggtggccccc gctgaattgg agtcgatatt gttacaacac cccaacatct
     1501 tcgacgcggg cgtggcaggt cttcccgacg atgacgccgg tgaacttccc gccgccgttg
     1561 ttgttttgga gcacggaaag acgatgacgg aaaaagagat cgtggattac gtcgccagtc
     1621 aagtaacaac cgccaaaaag ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag
     1681 gtcttaccgg aaaactcgac gcaagaaaaa tcagagagat cctcataaag gccaagaagg
     1741 gcggaaagtc caaattgtaa tgactctaga ggcatcaaat aaaacgaaag gctcagtcga
     1801 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa
     1861 atccgccgcc ctagacctag gggatatatt ccgcttcctc gctcactgac tcgctacgct
     1921 cggtcgttcg actgcggcga gcggaaatgg cttacgaacg gggcggagat ttcctggaag
     1981 atgccaggaa gatacttaac agggaagtga gagggccgcg gcaaagccgt ttttccatag
     2041 gctccgcccc cctgacaagc atcacgaaat ctgacgctca aatcagtggt ggcgaaaccc
     2101 gacaggacta taaagatacc aggcgtttcc ccctggcggc tccctcgtgc gctctcctgt
     2161 tcctgccttt cggtttaccg gtgtcattcc gctgttatgg ccgcgtttgt ctcattccac
     2221 gcctgacact cagttccggg taggcagttc gctccaagct ggactgtatg cacgaacccc
     2281 ccgttcagtc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggaaa
     2341 gacatgcaaa agcaccactg gcagcagcca ctggtaattg atttagagga gttagtcttg
     2401 aagtcatgcg ccggttaagg ctaaactgaa aggacaagtt ttggtgactg cgctcctcca
     2461 agccagttac ctcggttcaa agagttggta gctcagagaa ccttcgaaaa accgccctgc
     2521 aaggcggttt tttcgttttc agagcaagag attacgcgca gaccaaaacg atctcaagaa
     2581 gatcatctta ttaatcagat aaaatatttc tagatttcag tgcaatttat ctcttcaaat
     2641 gtagcacctg aagtcagccc catacgatat aagttgttac tagtgcttgg attctcacca
     2701 ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc
     2761 attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg ccccgccctg
     2821 ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca
     2881 gacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata
     2941 tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa
     3001 actggtgaaa ctcacccagg gattggctga gacgaaaaac atattctcaa taaacccttt
     3061 agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa
     3121 ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg
     3181 gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc
     3241 catacggaat tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata
     3301 aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt
     3361 ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca
     3421 ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc
     3481 tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg
     3541 aaagttggaa cctcttacgt gccgatcaac gtctcatttt cgccagatat cgacgtctaa
     3601 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     3661 cttcac
//
